| the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases |
RNA samples from the two groups (control and light-treated) were separated into two independent pools, each comprised of three distinct samples of equal amounts. Strand-specific libraries were constructed using a TruSeq RNA sample preparation kit (Illumina, San Diego, USA), and sequencing was carried out using an Illumina Novaseq 6000 instrument. The raw data was handled by Skewer, and data quality was checked by FastQC v0.11.2 (www.bioinformatics.babraham.ac.uk/projects/fastqc/). The read length was 2×150 bp. Clean reads were aligned to the Human genome hg38 using STAR |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
SINGLE
|
