| 实验编号 | CRX907628 |
| 物种名称 | Mus musculus |
| 标题 | DBIT-seq of mus musculus: E12.5 replicate2 |
| 项目编号 | PRJCA022162 |
| 样本编号 | SAMC3270881 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Tissues were collected for frozen sections. After quality control, tissue permeabilization, reverse transcription and two rounds of barcodes ligation were continuously conducted, then the reverse crosslinking were carried out. The lysate were purified and used for MyOne C1 beads binding to collecting cDNA and next template switch reaction. After amplification, the Novoprotein Tn5 enzyme were used to preduce random fragments. The 3 end products were amplified with illumina Nextera adapters and the libraries were sent for next generation sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-06-04 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR997278 |
CRR997278_f1.fq.gz
CRR997278_r2.fq.gz
|
3,017.34
2,357.31
|
|
| 提交者 | Junjie Lv (lv_junjie@gzlab.ac.cn) |
|---|
| 所属单位 | Guangzhou Laboratory |
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| 提交日期 | 2023-12-18 |
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