| 实验编号 | CRX912158 |
| 物种名称 | Gossypium hirsutum |
| 标题 | ZJ-3h-CK-2 |
| 项目编号 | PRJCA022415 |
| 样本编号 | SAMC3279971 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
| ZJ-3h-CK-2 |
RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina,following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2024-12-16 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1002021 |
CRR1002021_f1.fastq.gz
CRR1002021_r2.fastq.gz
|
2,106.42
2,203.79
|
|
| 提交者 | Xiongming Du (duxiongminglab@caas.cn) |
|---|
| 所属单位 | Institute of Cotton Research, Chinese Academy of Agricultural Sciences |
|---|
| 提交日期 | 2023-12-27 |
|---|
