| Accession | CRX912987 |
| Organism | Monochamus alternatus |
| Title | Mal_Hi-C |
| BioProject | PRJCA022378 |
| BioSample | SAMC3281316 |
| Platform | DNBSEQ-T7 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The tissue was vacuum infiltrated in nuclei isolation buffer supplemented with 2% formaldehyde. Adding glycine and additional vacuum infiltration to stop the crosslinking. Fixed tissue was grounded to powder and re-suspended in nuclei isolation buffer. The purified nuclei were digested with 100units of DpnII and marked by incubating with biotin-14-dATP. Removed biotin-14-dATP from non-ligated DNA ends using T4 DNA polymerase. The ligated DNA was sheared into 300-600 bp fragments, and then was blunt-end repaired and A-tailed, followed by purification through biotin-streptavidin-mediated pull down. |
Hi-C |
GENOMIC |
other |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-03-21 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1002984 |
CRR1002984_f1.fq.gz
CRR1002984_r2.fq.gz
|
53,122.34
50,890.63
|
|
| Submitter | Dunyang Yu (yukun7879@126.com) |
|---|
| Organization | Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences |
|---|
| Date submitted | 2023-12-28 |
|---|
