| Accession | CRX916291 |
| Organism | Oryza sativa |
| Title | D2_Mut1 |
| BioProject | PRJCA022639 |
| BioSample | SAMC3301664 |
| Platform | DNBSEQ-T10×4 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA for each three biological replicates consisted of panicles (approximately 10 cm in length, 5 individual plants per biological replicate) pooled from NIL-hws1WYJ7/hws2CG14 and NIL-HWS1CG14/hws2CG14 that were isolated independently after flash freezing in liquid nitrogen. The mRNA fraction was enriched using oligo (dT) magnetic beads and was uniformly fragmented to a specified length. First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by second-strand synthesis. The cDNA was next subjected to end-repair and the ends were 3’adenylated. Adapters were then ligated to the ends of 3’adenylated cDNA fragments. The cDNA fragments with adapters were then amplified and purified with Ampure XP Beads (Agencourt), and were dissolved in EB solution. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
SINGLE
|
|
| Processing |
Planned read length (bp): 50
|
| Release date | 2024-01-08 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1006609 |
CRR1006609.fq.gz
|
879.71
|
|
| Submitter | Ben Liao (liaoben@shanghaitech.edu.cn) |
|---|
| Organization | CAS Center for Excellence in Molecular Plant Sciences / Institute of Plant Physiology and Ecology |
|---|
| Date submitted | 2024-01-05 |
|---|
