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Experiment information
Accession CRX921112
Organism biofilm metagenome
Title D4_cKO6
BioProject PRJCA021383
BioSample SAMC3309524
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
DNA of sorted NCSCs was extracted from the cell line using a QIAamp DNA Mini Kit, sheared to approximately 300-500 bp using a Covaris S220 instrument. Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2024-01-15
Run
Run accession Run data file information
File nameFile size (MB)
CRR1011702 CRR1011702_f1.fq.gz
CRR1011702_r2.fq.gz
2,764.11
2,828.9
SubmitterLecheng Wei (lecwei@zju.edu.cn)
OrganizationZhejiang University
Date submitted2024-01-14
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Experiments(5)