| Accession | CRX924289 |
| Organism | Triticum aestivum |
| Title | CM104_HiFi |
| BioProject | PRJCA022052 |
| BioSample | SAMC3266712 |
| Platform | Pacbio Sequel II |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA (gDNA) was extracted from the seedling leaf tissues of CM104 according to the instructions for the CTAB method. Three methods were applied for DNA quantification and quality testing, namely, (i) NanoDrop 2000 spectrophotometry (Thermo Fisher Scientific), (ii) gel electrophoresis and (iii) Qubit fluorometry (Invitrogen). Total DNA was purified by AMPure PB beads (Pacbio 100-265-900). High-quality gDNA (>=10 μg, >=100 ng/μl) was prepared for the next step of library construction. PacBio single-molecule real-time (SMRT) bell library preparation was performed using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, PN 101-853-100) in accordance with the manufacturer's instructions (Procedure & Checklist - Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0). In brief, (i) 10 μg high-quality gDNA was evaluated using pulsed-field electrophoresis, and it was confirmed that most DNA fragments were longer than 40 kb. (ii) DNA was sheared to a mode size of 15 kb or larger using a Megaruptor instrument (Diagenode B06010001). (iii) AMPure PB Beads (Pacific Biosciences 100-265-900) were used to concentrate the DNA. (iv) The SMRTbell library was prepared using Kit 2.0. Single-strand overhangs were removed, and then we performed DNA damage repair, end repair, A-tailing, adapter ligation and enzymatic digestion. Finally, library size selection was conducted by SageELF (Sage Science ELF000) or the BluePippin System (Sage Science BLU0001). (v) After library size selection, the library was prepared for sequencing with a 30 h movie on the Sequel IIe system (Pacific Biosciences, CA, USA) |
WGS |
GENOMIC |
RANDOM |
SINGLE
|
|
| Processing |
|
| Release date | 2024-06-07 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1015107 |
CRR1015107.bam
|
26,913.93
|
| CRR1015108 |
CRR1015108.bam
|
24,229.47
|
| CRR1015109 |
CRR1015109.bam
|
26,548.8
|
| CRR1015110 |
CRR1015110.bam
|
24,378.14
|
| CRR1015111 |
CRR1015111.bam
|
22,802.43
|
| CRR1015112 |
CRR1015112.bam
|
19,287.34
|
| CRR1015113 |
CRR1015113.bam
|
28,986.71
|
| CRR1015114 |
CRR1015114.bam
|
28,096.79
|
| CRR1015115 |
CRR1015115.bam
|
25,502.26
|
| CRR1015116 |
CRR1015116.bam
|
26,339.96
|
| CRR1015117 |
CRR1015117.bam
|
24,514.05
|
| CRR1015118 |
CRR1015118.bam
|
26,391.78
|
| CRR1015119 |
CRR1015119.bam
|
28,027.96
|
| CRR1015120 |
CRR1015120.bam
|
25,774.51
|
| CRR1015121 |
CRR1015121.bam
|
23,161.45
|
| CRR1015122 |
CRR1015122.bam
|
26,646.4
|
| CRR1015123 |
CRR1015123.bam
|
23,185.47
|
| CRR1015124 |
CRR1015124.bam
|
24,229.51
|
| CRR1015125 |
CRR1015125.bam
|
21,720.35
|
| CRR1015126 |
CRR1015126.bam
|
23,074.94
|
|
| Submitter | Zehou Liu (zehouliu@163.com) |
|---|
| Organization | Sichuan Academy of Agricultural Sciences |
|---|
| Date submitted | 2024-01-18 |
|---|
