| 实验编号 | CRX958352 |
| 物种名称 | root metagenome |
| 标题 | Bulk soil from control for 16S amplicon sequencing ( bulk soil in in control replication 4 ) |
| 项目编号 | PRJCA023287 |
| 样本编号 | SAMC3332684 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
DNA for each sample were extracted by Power Soil DNA Isolation Kit (Qiagen, Germany), then the V3-V4 region of the 16S rDNA was alpliedied by two-step PCR,Library was prepared following the protocol of the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, USA). Finally, the amplicons were sequenced on Illumina Novaseq 6000 platform in pair-end 250 bp mode. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
| 发布日期 | 2024-02-04 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1050363 |
CRR1050363_f1.fq.gz
CRR1050363_r2.fq.gz
|
6.86
7.09
|
|
| 提交者 | zhenghong wang (wangzh@sustech.edu.cn) |
|---|
| 所属单位 | Southern University of Science and Technology |
|---|
| 提交日期 | 2024-02-04 |
|---|
