| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Approximately 200 mg of liver tissue was minced in 1 mL of PBS and cross-linked with 1% formaldehyde for 10 minutes, followed by quenching with glycine and lysis in the buffer. The cross-linked chromatin was sonicated to produce fragments of 100-300 bp, with 10 μL of the solution reserved as input. The remaining chromatin was then immunoprecipitated with an H3K27ac antibody (active motif, 39133), purified using magnetic beads and a column, and subjected to DNA sequencing with corresponding input samples using an Illumina HiSeq 2500 in a single-end model. |
ChIP-Seq |
GENOMIC |
ChIP |
SINGLE
|
|
