logo
Experiment information
Accession CRX960677
Organism Sus scrofa
Title Chip226
BioProject PRJCA022379
BioSample SAMC3369545
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Approximately 200 mg of liver tissue was minced in 1 mL of PBS and cross-linked with 1% formaldehyde for 10 minutes, followed by quenching with glycine and lysis in the buffer. The cross-linked chromatin was sonicated to produce fragments of 100-300 bp, with 10 μL of the solution reserved as input. The remaining chromatin was then immunoprecipitated with an H3K27ac antibody (active motif, 39133), purified using magnetic beads and a column, and subjected to DNA sequencing with corresponding input samples using an Illumina HiSeq 2500 in a single-end model. ChIP-Seq GENOMIC ChIP SINGLE
Processing Planned read length (bp): 50
Release date2024-03-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR1052854 CRR1052854.fq.gz 833.86
SubmitterZiqi Ling (827840800@qq.com)
OrganizationJiangxi Agricultural University
Date submitted2024-02-13
Related experiments
Experiments(291)