| Accession | CRX960697 |
| Organism | Sus scrofa |
| Title | Chip246 |
| BioProject | PRJCA022379 |
| BioSample | SAMC3369565 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Approximately 200 mg of liver tissue was minced in 1 mL of PBS and cross-linked with 1% formaldehyde for 10 minutes, followed by quenching with glycine and lysis in the buffer. The cross-linked chromatin was sonicated to produce fragments of 100-300 bp, with 10 μL of the solution reserved as input. The remaining chromatin was then immunoprecipitated with an H3K27ac antibody (active motif, 39133), purified using magnetic beads and a column, and subjected to DNA sequencing with corresponding input samples using an Illumina HiSeq 2500 in a single-end model. |
ChIP-Seq |
GENOMIC |
ChIP |
SINGLE
|
|
| Processing |
Planned read length (bp): 50
|
| Release date | 2024-03-13 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1052874 |
CRR1052874.fq.gz
|
1,110.03
|
|
| Submitter | Ziqi Ling (827840800@qq.com) |
|---|
| Organization | Jiangxi Agricultural University |
|---|
| Date submitted | 2024-02-13 |
|---|
