| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was isolated using TRIzol™ (Invitrogen, USA). The integrity and purity of RNA were tested by an eNanoPhotometer® spectrophotometer (IMPLEN, USA) and a Bioanalyzer 2100 system (Agilent Technologies, USA). Next, mRNA was enriched by poly-T oligo-attached magnetic beads in NEBNext® UltraTMR NA Library Prep Kit for Illumina (NEB, USA). Poly(A) + mRNA was then fragmented and used for strand-specific cDNA library construction. The cDNA was purified, end-repair, A-tailing, adaptor ligation and size selection using AMPure XP beads. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
