| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The starting RNA of the library was total RNA. mRNA with polyA tail was enriched by Oligo(dT) magnetic beads, and then the mRNA was randomly interrupted by divalent cations in Fragmentation Buffer. Using fragmented mRNA as template and random oligonucleotides as primer, the first cDNA strand was synthesized in M-MuLV reverse transcriptase system, then the RNA strand was degraded by RNaseH, and the second cDNA strand was synthesized in DNA polymerase I system using dNTPs as raw material. The purified double-stranded cDNA was end-repaired, A-tail was added, and sequencing joints were connected. cDNA of about 370~420 bp was screened by AMPure XP beads for PCR amplification, and PCR products were purified by AMPure XP beads again. Finally, A library was obtained. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
