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实验基本信息
实验编号 CRX968495
物种名称 Mus musculus
标题 AB4685
项目编号 PRJCA023228
样本编号 SAMC3391201
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Single cells were sorted based on specific staining (CD45) and transferred into 384-well capture plates. Briefly, Eeach well contained 2μL of lysis solution with barcoded ploy(T) reverse-transcription (RT) primers. To serve as the 'no-cell control', four empty wells (O1, O2, P1, P2) were included in each 385-well plate. The Massively Parallel Single-Cell RNA-seq method (MARS-seq) was used to prepare single-cell transcriptome libraries. RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA PAIRED
处理信息 Planned read length (bp) for mate 1: 75
Planned read length (bp) for mate 2: 15
发布日期2025-04-22
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1060849 CRR1060849_f1.fastq.gz
CRR1060849_r2.fastq.gz 		826.74
1,212.18
提交者hanjie li (ggwzs0217@163.com)
所属单位Shenzhen Institute of Advanced Technology
提交日期2024-02-22
关联实验
Experiments(25)