| 实验编号 | CRX968741 |
| 物种名称 | Triticum aestivum |
| 标题 | V28-ATAC_rep1 |
| 项目编号 | PRJCA023563 |
| 样本编号 | SAMC3394174 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
| V28-ATAC_rep1 |
The nuclei were isolated from the tissues and sorted with a flow cytometer (BD FacsAria II SORP). 50000 nuclei were collected and introduced into the tag-mentation reactions (Vazyme, #TD501) and DNA fragments from the last reactions were purified using the QIAGEN MinElute PCR Purification Kit (QIAGEN, #28006). High-Fidelity PCR Master Mix (NEB, M0541) was used to construct library with purified DNA. At last, the libraries were purified with AMPure beads and quality detected with Agilent 2100 Bioanalyzer. Subsequently, the good quality libraries were sequenced by the NovaSeq 6000 system to produce the 150-bp paired-end reads. |
ATAC-seq |
GENOMIC |
other |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2024-11-02 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1061106 |
CRR1061106_f1.fq.gz
CRR1061106_r2.fq.gz
|
1,630.73
1,666.05
|
|
| 提交者 | CHAO HE (hechao7021@163.com) |
|---|
| 所属单位 | Huazhong Agricultural University |
|---|
| 提交日期 | 2024-02-23 |
|---|
