| Accession | CRX990566 |
| Organism | Brassica rapa subsp. oleifera |
| Title | L5-1 |
| BioProject | PRJCA024386 |
| BioSample | SAMC3447477 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
After the sample test is qualified, the library is constructed. The main process is as follows:
(1) Enrichment of eukaryotic mRNA with magnetic beads with Oligo (dT);
(2) mRNA was randomly interrupted by Fragmentation Buffer.
(3) Using mRNA as template, the first cDNA chain and two strands were synthesized, and the cDNA was purified;
(4) The purified double-stranded cDNA was then end-repaired, A tail was added, and sequencing joints were connected, and then slices were made with AMPure XP beads
Segment size selection;
(7) cDNA library was obtained by PCR enrichment. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-03-18 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1083783 |
CRR1083783_f1.fq.gz
CRR1083783_r2.fq.gz
|
1,593.69
1,651.06
|
|
| Submitter | Xuelian Ye (yexuelian201@163.com) |
|---|
| Organization | College of Agriculture and Biotechnology, Zhejiang University |
|---|
| Date submitted | 2024-03-17 |
|---|
