| 实验编号 | CRX1003349 |
| 物种名称 | Silurus lanzhouensis |
| 标题 | HiC_YY |
| 项目编号 | PRJCA016180 |
| 样本编号 | SAMC3476255 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Chromatin-associated proteins in samples were cross-linked to DNA with 1% formaldehyde and quenched with glycine. Subsequently, the fixed cells were lysed and nuclei suspension was generated. The DNA in nuclei mixture was digested with MboI restriction endonuclease and sticky ends were repaired with biotin-14-dCTP by incubating with Klenow enzyme. Next, obtained blunt-end-repaired DNA strands were performed proximity ligation by T4 DNA polymerase and the cross-linked proteins were digested with proteinase K. Finally, the remaining chromatin DNA was purified and sheared to fragments with length of ~350 bp, and then the Hi-C library for Illumina sequencing was prepared after adapters were ligated onto the 3 end of the sheared fragments and PCR. The Hi- C library was constructed using with NEBNext Ultra II DNA library Prep Kit (NEB,USA) |
Hi-C |
GENOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2024-04-06 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1083936 |
CRR1083936_f1.fq.gz
CRR1083936_r2.fq.gz
|
22,326.97
24,663.92
|
|
| 提交者 | Tao Wang (wangtao01@ihb.ac.cn) |
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| 所属单位 | Institute of Hydrobiology, Chinese Academy of Sciences |
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| 提交日期 | 2024-03-25 |
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