logo
Experiment information
Accession CRX1003790
Organism Paeonia lactiflora
Title white3
BioProject PRJCA024685
BioSample SAMC3479607
Platform BGISEQ-500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using CTAB-LiCl method, and genomic DNA contamination was removed using DNase I. mRNA was enriched with oligo (dT) magnetic beads and then broken into smaller pieces using fragmentation buffer. The first strand cDNA was reversed-transcribed by random hexamers and small fragment as templates. This was followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The double strand cDNA was ligated to paired-end adapters, and suitable fragments were selected and enriched with PCR amplification. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Release date2024-04-03
Run
Run accession Run data file information
File nameFile size (MB)
CRR1091092 CRR1091092_f1.fq.gz
CRR1091092_r2.fq.gz
2,758.86
2,916.06
SubmitterZhang Yanzhao (landscape031@163.com)
OrganizationLuoyang Normal University
Date submitted2024-03-27
Related experiments
Experiments(4)