| Accession | CRX1003793 |
| Organism | Paeonia lactiflora |
| Title | red3 |
| BioProject | PRJCA024685 |
| BioSample | SAMC3479610 |
| Platform | BGISEQ-500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted using CTAB-LiCl method, and genomic DNA contamination was removed using DNase I. mRNA was enriched with oligo (dT) magnetic beads and then broken into smaller pieces using fragmentation buffer. The first strand
cDNA was reversed-transcribed by random hexamers and small fragment as templates. This was followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The double strand cDNA was ligated to paired-end adapters, and suitable fragments were selected and enriched with PCR amplification. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
|
| Release date | 2024-04-03 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1091095 |
CRR1091095_f1.fq.gz
CRR1091095_r2.fq.gz
|
2,780.38
2,945.13
|
|
| Submitter | Zhang Yanzhao (landscape031@163.com) |
|---|
| Organization | Luoyang Normal University |
|---|
| Date submitted | 2024-03-27 |
|---|
