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For the HiFi SMRTbell library, 8 μg of DNA was used to prepare library using the SMRTbell Express Template Preparation Kit 2.0 (Pacific Biosciences, USA) following the manufacturer's recommendations. The library was sequenced using the HiFi SMRTbell sequencing method with an average fragment size of 15 to 20 kb. The DNA library was loaded onto the flow cell with sequencing buffer and loading beads, and sequencing was performed using the DNA Sequencing Reagent Kit (Pacific Biosciences, USA) according to the manual. |
WGS |
GENOMIC |
RANDOM |
SINGLE
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