Experiment information
Accession CRX1005127
Organism Populus simonii
Title Populus simonii XY4 leaf RNA-seq 1
BioProject PRJCA024732
BioSample SAMC3482253
Platform Ion Torrent Proton
Library name Construction protocol Strategy Source Selection Layout
cDNA The cDNA libraries for single-end sequencing were prepared using Ion Total RNA-Seq Kit v2.0 (Life Technologies) according to the manufacturer’s instructions. The cDNA libraries were then processed for the Proton Sequencing process according to the commercially available protocols. Samples were diluted and mixed, the mixture was processed on a OneTouch 2 instrument (Life Technologies) and enriched on a OneTouch 2 ES station (Life Technologies) for preparing the template-positive Ion PI™ Ion Sphere™ Particles (Life Technologies) according to Ion PI™ Template OT2 200 Kit v2.0 (Life Technologies). After enrichment, the mixed template-positive Ion PI™ Ion Sphere™ Particles of samples were loaded onto 1 P1v2 Proton Chip (Life Technologies) and sequenced on Proton Sequencers according to Ion PI Sequencing 200 Kit v2.0 (Life Technologies). RNA-Seq TRANSCRIPTOMIC unspecified SINGLE
Release date2024-04-03
Run accession Run data file information
File nameFile size (MB)
CRR1092442 CRR1092442.fq.gz 4,534.04
SubmitterJun Wang (wangjun@bjfu.edu.cn)
OrganizationBeijing Forestry University
Date submitted2024-03-28
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