| 实验编号 | CRX1005215 |
| 物种名称 | Populus |
| 标题 | Populus hybrid_2 leaf RNA-seq 1 |
| 项目编号 | PRJCA024732 |
| 样本编号 | SAMC3483390 |
| 测序平台 | Ion Torrent Proton |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
| cDNA |
The cDNA libraries for single-end sequencing were prepared using Ion Total RNA-Seq Kit v2.0 (Life Technologies) according to the manufacturer’s instructions. The cDNA libraries were then processed for the Proton Sequencing process according to the commercially available protocols. Samples were diluted and mixed, the mixture was processed on a OneTouch 2 instrument (Life Technologies) and enriched on a OneTouch 2 ES station (Life Technologies) for preparing the template-positive Ion PI™ Ion Sphere™ Particles (Life Technologies) according to Ion PI™ Template OT2 200 Kit v2.0 (Life Technologies). After enrichment, the mixed template-positive Ion PI™ Ion Sphere™ Particles of samples were loaded onto 1 P1v2 Proton Chip (Life Technologies) and sequenced on Proton Sequencers according to Ion PI Sequencing 200 Kit v2.0 (Life Technologies). |
RNA-Seq |
TRANSCRIPTOMIC |
unspecified |
SINGLE
|
|
| 处理信息 |
|
| 发布日期 | 2024-04-03 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1092530 |
CRR1092530.fq.gz
|
5,129.83
|
|
| 提交者 | Jun Wang (wangjun@bjfu.edu.cn) |
|---|
| 所属单位 | Beijing Forestry University |
|---|
| 提交日期 | 2024-03-28 |
|---|
