| Accession | CRX1005217 |
| Organism | Populus |
| Title | Populus hybrid_2 leaf RNA-seq 3 |
| BioProject | PRJCA024732 |
| BioSample | SAMC3483392 |
| Platform | Ion Torrent Proton |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| cDNA |
The cDNA libraries for single-end sequencing were prepared using Ion Total RNA-Seq Kit v2.0 (Life Technologies) according to the manufacturer’s instructions. The cDNA libraries were then processed for the Proton Sequencing process according to the commercially available protocols. Samples were diluted and mixed, the mixture was processed on a OneTouch 2 instrument (Life Technologies) and enriched on a OneTouch 2 ES station (Life Technologies) for preparing the template-positive Ion PI™ Ion Sphere™ Particles (Life Technologies) according to Ion PI™ Template OT2 200 Kit v2.0 (Life Technologies). After enrichment, the mixed template-positive Ion PI™ Ion Sphere™ Particles of samples were loaded onto 1 P1v2 Proton Chip (Life Technologies) and sequenced on Proton Sequencers according to Ion PI Sequencing 200 Kit v2.0 (Life Technologies). |
RNA-Seq |
TRANSCRIPTOMIC |
unspecified |
SINGLE
|
|
| Processing |
|
| Release date | 2024-04-03 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1092532 |
CRR1092532.fq.gz
|
4,535.34
|
|
| Submitter | Jun Wang (wangjun@bjfu.edu.cn) |
|---|
| Organization | Beijing Forestry University |
|---|
| Date submitted | 2024-03-28 |
|---|
