| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
cell nuclei were first extracted from young leaves (1.5 g) of a single one-year-old plant as previously described (Weng et al., 2018). Nuclear DNA was isolated using CTAB extraction buffer (100 mM Tris-HCl, 25 mM EDTA, 1.5 M NaCl, and 3% CTAB). DNA libraries for single-molecule real-time (SMRT) PacBio genome sequencing were constructed following standard protocols (Pacific Biosciences, Menlo Park, CA, USA). Briefly, genomic DNA was sheared to ~20-kb targeted size, followed by damage repair and end repair, blunt-end adaptor ligation, and size selection. Finally, the libraries were sequenced on the PacBio Sequel II platform |
WGS |
GENOMIC |
RANDOM |
SINGLE
|
|
