| 实验编号 | CRX1007723 |
| 物种名称 | Ricinus communis |
| 标题 | L-2 |
| 项目编号 | PRJCA024857 |
| 样本编号 | SAMC3487115 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total RNA was extracted using Trizol reagent kit according to the manufacturer's protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer and checked using RNase free agarose gelelectrophoresis. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina. The purified double-stranded cDNA fragments were end repaired, A base added, and ligated to Illumina sequencing adapters. The ligation reaction was purified with the AMPure XP Beads And polymerase chain reaction amplified. The resulting cDNA library was sequenced using Illumina Novaseq6007 by Gene Denovo Biotechnology Co. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2024-04-03 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1095142 |
CRR1095142_f1.fq.gz
CRR1095142_r2.fq.gz
|
1,270.5
1,289.37
|
|
| 提交者 | Siyu Chen (chensyv@163.com) |
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| 所属单位 | Central South University of Forestry and Technology |
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| 提交日期 | 2024-04-02 |
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