| Accession | CRX1007727 |
| Organism | Ricinus communis |
| Title | LI-3 |
| BioProject | PRJCA024857 |
| BioSample | SAMC3487119 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted using Trizol reagent kit according to the manufacturer's protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer and checked using RNase free agarose gelelectrophoresis. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina. The purified double-stranded cDNA fragments were end repaired, A base added, and ligated to Illumina sequencing adapters. The ligation reaction was purified with the AMPure XP Beads And polymerase chain reaction amplified. The resulting cDNA library was sequenced using Illumina Novaseq6011 by Gene Denovo Biotechnology Co. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-04-03 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1095146 |
CRR1095146_f1.fq.gz
CRR1095146_r2.fq.gz
|
1,186.73
1,214.73
|
|
| Submitter | Siyu Chen (chensyv@163.com) |
|---|
| Organization | Central South University of Forestry and Technology |
|---|
| Date submitted | 2024-04-02 |
|---|
