| 实验编号 | CRX1022845 |
| 物种名称 | Macaca mulatta |
| 标题 | LCL8664_RNAPII_ChIA-PET _replicate 3 |
| 项目编号 | PRJCA020156 |
| 样本编号 | SAMC3516693 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Ten million B cells were crosslined, lysed to permeabilize cell membrane, and treated with AluI to digest DNA. The DNA ends were A-tailed and DNA ends in the proximity were then ligated to the same biotinylated linkers. After sonication, 20 microgram of RNAPII antibody were applied to capture RNAPII binding chromatin. The ChIP DNA were decrosslinked, purified, and Tn5 tagmented, and then the DNAs with biotin were captured on streptatvidin beads. The DNAs on beads were then subjected to index PCR for libarary construction. |
ChIA-PET |
GENOMIC |
ChIP |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2024-12-12 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1113466 |
CRR1113466_f1.fq.gz
CRR1113466_r2.fq.gz
|
1,253.91
1,236.74
|
| CRR1113467 |
CRR1113467_f1.fq.gz
CRR1113467_r2.fq.gz
|
9,670.39
9,748.02
|
| CRR1113468 |
CRR1113468_f1.fq.gz
CRR1113468_r2.fq.gz
|
5,122.09
5,324.75
|
| CRR1113469 |
CRR1113469_f1.fq.gz
CRR1113469_r2.fq.gz
|
9,990.73
10,071.77
|
|
| 提交者 | Zhonghui Tang (tangzhh99@mail.sysu.edu.cn) |
|---|
| 所属单位 | Sun Yat-sen University |
|---|
| 提交日期 | 2024-04-16 |
|---|
