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Fifty ng of the 5’-end scRNA-seq cDNAs derived from rhesus macaque primary neuronal cells cultured at day 15 were first amplified by PCR for 12 cycles with the PR1 primer on the 5’-end of each cDNA fragment and a specific 3’-end primer that targetes to the unique exons of the DLGAP1-206 isoform at the 5’ end of the isoform. The PCR products were purified and applied for a ten-cycle nested-PCR amplification using the PR1 primer and another 3’-end primer that targets to an inner position at the unique exons of the DLGAP1-206 isoform. Later, sequencing adapters and indexes were added to the amplified TSS DNA fragments through PCR. |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
PCR |
SINGLE
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