| 实验编号 | CRX1024202 |
| 物种名称 | Saccharomyces cerevisiae |
| 标题 | m13-1 |
| 项目编号 | PRJCA025349 |
| 样本编号 | SAMC3528523 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
After the samples were qualified, the library was constructed: eukaryotic mRNA was enriched with magnetic beads with Oligo (dT); mRNA was randomly interrupted by Fragmentation Buffer. Using mRNA as template, the first cDNA strand and two cDNA strands were synthesized, and the cdna was purified. The purified double-stranded cDNA was then end-repaired, A tail was added and sequencing joints were connected, and the fragment size was selected by AMPure XP beads. Finally, cDNA library was obtained by PCR enrichment. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| 发布日期 | 2024-09-24 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1114876 |
CRR1114876_f1.fq.gz
CRR1114876_r2.fq.gz
|
1,633.73
1,749.17
|
|
| 提交者 | Dong Lu (ld@impcas.ac.cn) |
|---|
| 所属单位 | Institute of Modern Physics, Chinese Academy of Sciences |
|---|
| 提交日期 | 2024-04-17 |
|---|
