| 实验编号 | CRX1024255 |
| 物种名称 | Severe acute respiratory syndrome coronavirus 2 |
| 标题 | 5 |
| 项目编号 | PRJCA025203 |
| 样本编号 | SAMC3528470 |
| 测序平台 | NextSeq CN500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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DNA was extracted from all samples using a QIAamp® UCP Pathogen DNA Kit (Qiagen) following the manufacturer’s instructions. Human DNA was removed using Benzonase (Qiagen) and Tween20 (Sigma). Total RNA was extracted with a QIAamp® Viral RNA Kit (Qiagen) and ribosomal RNA was removed by a Ribo-Zero rRNA Removal Kit (Illumina). cDNA was generated using reverse transcriptase and dNTPs (Thermo Fisher). Libraries were constructed for the DNA and cDNA samples using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA)2. Library was quality assessed by Qubit dsDNA HS Assay kit followed by High Sensitivity DNA kit (Agilent) on an Agilent 2100 Bioanalyzer. Library pools were then loaded onto an Illumina Nextseq CN500 sequencer for 75 cycles of single-end sequencing to generate approximately 20 million reads for each library. For negative controls, we also prepared PBMC samples with 105 cells/mL from healthy donors in parallel with each batch, using the same protocol, and sterile deionized water was extracted alongside the specimens to serve as non-template controls (NTC). |
OTHER |
METAGENOMIC |
RANDOM |
SINGLE
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| 处理信息 |
Planned read length (bp): 50
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| 发布日期 | 2024-04-18 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR1114929 |
CRR1114929.fastq.gz
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159.46
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| 提交者 | Yuan Yang (yangyuan361@126.com) |
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| 所属单位 | Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital |
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| 提交日期 | 2024-04-17 |
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