| 实验编号 | CRX1045587 |
| 物种名称 | soil metagenome |
| 标题 | TB3-2 |
| 项目编号 | PRJCA025605 |
| 样本编号 | SAMC3572200 |
| 测序平台 | Illumina MiSeq |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Soil genomic DNA extraction was conducted using the E.Z.N.A. Soil DNA Kit (Omega Bio-tek, Inc., USA) according to the manufacturer's instructions. The concentration and quality of the genomic DNA were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific Inc., USA). Subsequently, DNA samples were stored at -20°C for further experimentation. The V3-4 hypervariable region of the bacterial 16S rRNA gene was amplified using the universal primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’) and 806R (5’-GGACTACNNGGGTATCTAAT-3’). Similarly, the internal transcribed spacer 1 (ITS1) region of the fungal ribosomal RNA (rRNA) gene was targeted using the primers ITS1 (5’-CTTGGTCATTTAGAGGAAGTAA-3’) and ITS2 (5’-TGCGTTCTTCATCGATGC-3’). |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
|
| 发布日期 | 2024-07-02 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1137338 |
CRR1137338_f1.fq.gz
CRR1137338_r2.fq.gz
|
12.1
12.58
|
|
| 提交者 | LiLi FAN (yfllyin@163.com) |
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| 所属单位 | Research Institute of Subtropical Forestry, Chinese Academy of Forestry |
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| 提交日期 | 2024-05-06 |
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