| 实验编号 | CRX1048327 |
| 物种名称 | Nicotiana tabacum |
| 标题 | transcriptomic analysis of tobacco plant overexpressing empty vector R4 |
| 项目编号 | PRJCA025907 |
| 样本编号 | SAMC3704794 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
poly(A)+ RNA was purified from 20 μg pooled total RNA by using oligo(dT) magnetic beads. Fragmentation was implemented in the presence of divalent cations at 94 °C for 5 min. Then, N6 random primers were used for reverse transcription into the double-stranded complementary DNA (cDNA). After end-repair and adaptor ligation, the products were amplified by PCR and purified using a QIAquick PCR purification kit |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 350
Planned read length (bp) for mate 2: 350
Insert size (bp): 350
|
| 发布日期 | 2026-03-25 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1140468 |
CRR1140468_f1.fastq.gz
CRR1140468_r2.fastq.gz
|
1,240.08
1,310.31
|
|
| 提交者 | Hai-Jian Huang (huanghaijian@nbu.edu.cn) |
|---|
| 所属单位 | Ningbo University |
|---|
| 提交日期 | 2024-05-07 |
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