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Genomic DNA was extracted using a Cetyltrimethylam-Monium bromide (CTAB)-based protocol. DNA was randomly broken into fragments of 200-500 bp size by mechanical interruption (ultrasound), then fragment purification, end repair, 3 'end addition of A, connection of sequencing joints were performed on the fragmented DNA, fragment size selection was performed by agarose gel electrophoresis, and PCR amplification was performed to form sequencing library. |
WGS |
GENOMIC |
RANDOM |
PAIRED
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