| Accession | CRX1145868 |
| Organism | Capra hircus |
| Title | siNC-3 |
| BioProject | PRJCA028328 |
| BioSample | SAMC4034357 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Novaseq 6000 platform and 150 bp paired-end reads were generated. |
RNA-Seq |
TRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-11-30 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1241627 |
CRR1241627_f1.fq.gz
CRR1241627_r2.fq.gz
|
1,915.34
2,011.22
|
|
| Submitter | Zihao Zhao (z15528133630@163.com) |
|---|
| Organization | Sichuan Agriculture University |
|---|
| Date submitted | 2024-07-23 |
|---|
