| 实验编号 | CRX1145899 |
| 物种名称 | Juglans regia |
| 标题 | A3_3 |
| 项目编号 | PRJCA028432 |
| 样本编号 | SAMC4039523 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
The initial RNA was total RNA, total RNA > = 1ug. The library building kit used in library building is Illumina NEBNext(R) UltraTM RNA Library Prep Kit. The mRNA with polyA tail was enriched by Oligo ( dT ) beads, and then the mRNA was randomly interrupted by divalent cations in NEB Fragmentation Buffer. The first strand of cDNA was synthesized in M-MuLV reverse transcriptase system with random oligonucleotide primers and fragmentized mRNA as template. RNaseH degrades RNA strand and synthesizes dNTPs in DNA polymerase I system Make the second strand of cDNA. Purified double-stranded cDNA was repaired by terminal repair Add A tail and connect the sequencing joint, and use AMPure XP beads to screen about 250 ~ 300 bp cDNA, amplified by PCR and purified by AMPure XP beads, Finally, the library was obtained. |
OTHER |
TRANSCRIPTOMIC |
size fractionation |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2024-08-10 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1241658 |
CRR1241658_f1.fq.gz
CRR1241658_r2.fq.gz
|
1,450.78
1,477.01
|
|
| 提交者 | Shugang Zhao (zshug@126.com) |
|---|
| 所属单位 | Hebei Agricultural University |
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| 提交日期 | 2024-07-24 |
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