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实验基本信息
实验编号 CRX1165864
物种名称 Fusarium graminearum
标题 chip-seq of Rlm1_TBI_IP_1
项目编号 PRJCA029352
样本编号 SAMC4086638
测序平台 Illumina HiSeq 2500
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
FgRlm1-GFP strains were cultured in TBI medium for 24 h. Fresh mycelia of each strain were cross-linked with 1% formaldehyde for 15 min and then stopped with 125 mM glycine.The DNAs were sheared into 200-500 bp fragments by a Bioruptor Plus (Diagenode, UCD-300) with 30s on and 30s off. The supernatant of each sample was mixed with the rabbit polyclonal anti-GFP (ab290, Abcam, Cambridge, UK;1:500 dilution) antibody ChIP-Seq GENOMIC ChIP PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2024-10-22
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1261561 CRR1261561.bam 231.42
提交者Xu chaoyun (424347175@qq.com)
所属单位Zhejiang University
提交日期2024-08-22
关联实验
Experiments(3)