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实验基本信息
实验编号 CRX1168091
物种名称 Oryza sativa
标题 Mock_2_IP_m6A
项目编号 PRJCA021138
样本编号 SAMC4095423
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Mock_2_IP_m6A or each library, mRNAs were enriched from total RNA using the VAHTS mRNA Capture Beads. The mRNA samples were fragmented with 20 mM ZnCl2 for 5 min until RNAs fragments were mainly distributed between 100-250 nt. Then RNA fragments of 10% and the rest were used for input immunoprecipitation (IP) respectively. The specific antibodies of anti-m6A were applied to immunoprecipitation for m1A-RIP-seq RIP-Seq TRANSCRIPTOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2024-08-27
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1263284 CRR1263284_f1.fastq.gz
CRR1263284_r2.fastq.gz
391.28
488.85
提交者Xiang Lu (allen-luxiang@163.com)
所属单位Sichuan Agricultural University
提交日期2024-08-24