| 实验编号 | CRX1168091 |
| 物种名称 | Oryza sativa |
| 标题 | Mock_2_IP_m6A |
| 项目编号 | PRJCA021138 |
| 样本编号 | SAMC4095423 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
| Mock_2_IP_m6A |
or each library, mRNAs were enriched from total RNA using the VAHTS mRNA Capture Beads. The mRNA samples were fragmented with 20 mM ZnCl2 for 5 min until RNAs fragments were mainly distributed between 100-250 nt. Then RNA fragments of 10% and the rest were used for input immunoprecipitation (IP) respectively. The specific antibodies of anti-m6A were applied to immunoprecipitation for m1A-RIP-seq |
RIP-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2024-08-27 |
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
| CRR1263284 |
CRR1263284_f1.fastq.gz
CRR1263284_r2.fastq.gz
|
391.28
488.85
|
|
| 提交者 | Xiang Lu (allen-luxiang@163.com) |
| 所属单位 | Sichuan Agricultural University |
| 提交日期 | 2024-08-24 |