| Accession | CRX1177019 |
| Organism | Mus musculus |
| Accession in Other Database |
: |
| Title | W7_cKO2 |
| BioProject | PRJCA029538 |
| BioSample | SAMC4108874 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The library preparation was conducted following the manufacturer’s instructions (10x Genomics). Initially, total cells were resuspended in the master mix and co-loaded with partitioning oil and gel beads. Subsequently, the poly-A RNA extracted from the cell lysate was reverse-transcribed into cDNA, incorporating an Illumina R1 primer sequence, a unique molecular identifier (UMI), and the 14x Barcode. The barcoded cDNA pool was then purified, PCR-amplified, and appropriately-sized fragments were selected using SPRIselect reagent for subsequent library construction. |
RNA-Seq |
GENOMIC SINGLE CELL |
cDNA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2024-10-08 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1272318 |
CRR1272318_f1.fastq.gz
CRR1272318_r2.fastq.gz
|
7,195.81
16,782.09
|
|
| Submitter | Hongzhong Li (lihongzhong@cqmu.edu.cn) |
|---|
| Organization | The First Affiliated Hospital of Chongqing Medical University |
|---|
| Submitted date | 2024-08-30 |
|---|
