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Genomic DNA was extracted using TIANamp genomic DNA kits (Tiangen Ltd, Beijing). Adapter ligation and clean up was performed using MGIEasy DNA Adapters kits and PCR was carried out on purified adapter-ligated DNA and cleaned-up again using magnetic beads. After quality control using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MD, USA), purified PCR products were denatured and ligated to generate single-strand circular DNA libraries. |
WGS |
GENOMIC |
RANDOM |
PAIRED
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