| 实验编号 | CRX1189554 |
| 物种名称 | Nymphaea |
| 标题 | C2_Ctrl12_1 |
| 项目编号 | PRJCA030030 |
| 样本编号 | SAMC4131327 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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High-quality RNA was extracted, purity, concentration and integrity were tested, and mRNA was enriched by mRNA Capture Beads(oligo dT magnetic beads). RNA fragmentation; cDNA first strand synthesis; cDNA second strand synthesis; ds cDNA end repair and dA-tailing; Connecting sequencing adapter; Magnetic bead purification or sorting; PCR amplification (option); PCR product concentration and fragment size distribution were detected. Degeneration and cyclization of PCR products; Digestion of linear products by enzyme digestion; pooling is carried out according to the target data quantity; The high-throughput sequencing platform DNBSEQ was used for sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2024-10-24 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1295182 |
CRR1295182_f1.fq.gz
CRR1295182_r2.fq.gz
|
2,960.3
2,974.36
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|
| 提交者 | Fei Chen (810569243@qq.com) |
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| 所属单位 | Hainan University |
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| 提交日期 | 2024-09-12 |
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