| 实验编号 | CRX1279321 |
| 物种名称 | Mus musculus |
| 标题 | CN_4 |
| 项目编号 | PRJCA030100 |
| 样本编号 | SAMC4415665 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Feces and saliva underwent DNA extraction using the CTAB method as per the manufacturer’s instructions44. The extracted total DNA was eluted in the elution buffer and subjected to PCR analysis. Subsequently, the samples were sequenced on an Illumina NovaSeq platform according to the manufacturer’s instructions. Paired-end reads were sorted based on their unique barcode and then trimmed by removing the barcode and primer sequence. FLASH was utilized to merge the paired-end reads. Quality filtering of the raw reads was performed under specific conditions to obtain high-quality clean tags using fqtrim. Chimeric sequences were eliminated using Vsearch software. After dereplication using DADA2, a ASV feature table and feature sequence were obtained. Feature sequences were annotated with SILVA database45 |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2026-03-03 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1395441 |
CRR1395441_r1.fastq.gz
CRR1395441_r2.fastq.gz
|
3.53
3.78
|
|
| 提交者 | Fengqing Lin (602022350068@smail.nju.edu.cn) |
|---|
| 所属单位 | Nanjing University |
|---|
| 提交日期 | 2024-11-29 |
|---|
