| 实验编号 | CRX1200449 |
| 物种名称 | Mus musculus |
| 标题 | W7_cKO1 |
| 项目编号 | PRJCA030466 |
| 样本编号 | SAMC4153381 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
The library preparation was conducted following the manufacturer’s instructions (10x Genomics). Initially, total cells were resuspended in the master mix and co-loaded with partitioning oil and gel beads. Subsequently, the poly-A RNA extracted from the cell lysate was reverse-transcribed into cDNA, incorporating an Illumina R1 primer sequence, a unique molecular identifier (UMI), and the 10x Barcode. The barcoded cDNA pool was then purified, PCR-amplified, and appropriately-sized fragments were selected using SPRIselect reagent for subsequent library construction. |
RNA-Seq |
GENOMIC SINGLE CELL |
cDNA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| 发布日期 | 2025-05-04 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1309596 |
CRR1309596_f1.fastq.gz
CRR1309596_r2.fastq.gz
|
6,703.91
15,856.04
|
|
| 提交者 | Hongzhong Li (lihongzhong@cqmu.edu.cn) |
|---|
| 所属单位 | The First Affiliated Hospital of Chongqing Medical University |
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| 提交日期 | 2024-09-24 |
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