| 实验编号 | CRX1202415 |
| 物种名称 | Mus musculus |
| 标题 | vitroEBsncRNApool2 |
| 项目编号 | PRJCA030502 |
| 样本编号 | SAMC4157765 |
| 测序平台 | MGISEQ-2000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total RNA from blastocysts was isolated and enriched for polyadenylated mRNA using Oligo(dT) magnetic beads, followed by reverse transcription and amplification according to the Smart-seq2 protocol. The resulting double-stranded complementary DNA (cDNA) was treated with Tn5 transposase for fragmentation and adapter ligation, constructing libraries suitable for high-throughput sequencing. Library quality was assessed using an Agilent 2100 Bioanalyzer and quantitative PCR (qPCR) before sequencing on the DNBSEQ platform, generating paired-end 100 base pair (PE100) reads, providing a minimum of 16 gigabytes (GB) of high-quality data per sample. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
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| 发布日期 | 2025-07-26 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1311933 |
CRR1311933_f1.fq.gz
CRR1311933_r2.fq.gz
|
3,464.51
3,423.57
|
|
| 提交者 | Ying Zhang (zhangyinglab@bnu.edu.cn) |
|---|
| 所属单位 | Beijing Normal University |
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| 提交日期 | 2024-09-27 |
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