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实验基本信息
实验编号 CRX1214937
物种名称 Begonia fimbristipula
标题 Bfim_HiC
项目编号 PRJCA031018
样本编号 SAMC4231187
测序平台 Illumina HiSeq X Ten
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Young and fresh leaves were fixed in a nuclei isolation buffer with 2% formaldehyde, then cross-linked DNA was digested with 100 units of DpnII (New England Biolabs, USA). The digested fragments were biotinylated with biotin-14-dCTP and ligated using T4 DNA polymerase (New England Biolabs). The ligated DNA was enriched, sheared into 300- to 600-bp fragments, blunt-end repaired, and further processed. WGS GENOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2024-10-25
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1328620 CRR1328620_r1.fastq.gz
CRR1328620_r2.fastq.gz
46,408.41
47,929.7
提交者TW Xiao (928162526@qq.com)
所属单位South China Botanical Garden, Chinese Academy of Sciences
提交日期2024-10-11
关联实验
Experiments(4)