| Accession | CRX1222954 |
| Organism | Chlamydomonas reinhardtii |
| Title | ETP_BP0_3 |
| BioProject | PRJCA031121 |
| BioSample | SAMC4251774 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Extract total RNA from tissue samples, measure the concentration and purity of the extracted RNA using Nanodrop2000, assess RNA integrity via agarose gel electrophoresis, and determine the RQN value with Agilent5300. Eukaryotic mRNA features a poly(A) tail at the 3' end, which allows separation of mRNA from total RNA through A-T base pairing with Oligo(dT)-coated magnetic beads. By adding fragmentation buffer, the enriched mRNA is randomly fragmented into small segments of approximately 300 bp. Using random primers and mRNA as a template, first-strand cDNA is synthesized by reverse transcription. This is followed by second-strand synthesis to form a stable double-stranded structure. The double-stranded cDNA has sticky ends, which are converted to blunt ends by adding End Repair Mix. An A base is then added to the 3' end to facilitate subsequent adapter ligation. The adapter-ligated products undergo purification and size selection. The selected fragments are PCR-amplified, and the final library is purified. |
RNA-Seq |
TRANSCRIPTOMIC |
other |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-10-15 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1336683 |
CRR1336683_r1.fastq.gz
CRR1336683_r2.fastq.gz
|
2,014.3
2,079.57
|
|
| Submitter | WEI HE (wei.he@cugb.edu.cn) |
|---|
| Organization | China University of Geosciences Beijing |
|---|
| Date submitted | 2024-10-14 |
|---|
