| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Briefly, about 3 to 5 g of young leaves were collected and used for nuclei extraction. Extracted nuclei were digested with MNase (New England BioLabs, 0.5 U) at 37℃ for 20 min. Then, oat CENH3 antibody was prepared for ChIP experiments. Subsequently, Dynabeads Protein G (Invitrogen, Carlsbad, CA, USA) was used to capture the target antibody, and bound chromatin was eluted, de-crosslinked, and precipitated. The ChIPed DNA and input control DNA were used to generate the ChIP-seq library using the NadPrep DNA Library Preparation Kit for Illumina (1002101) and NadPrep UDI Adapter Kit Set C1 for Illumina (1003221), following the manufacturer’s recommendations. Libraries were sequenced on an Illumina NovaSeq6000 in 150-bp PE mode. |
ChIP-Seq |
GENOMIC |
ChIP |
PAIRED
|
|
