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Cells are fixed with formaldehyde to cross-link DNA with proteins and proteins with each other. After cross-linking, cell lysis is performed, and a sample is taken for quality assessment. If the quality is acceptable, the process proceeds to the "Hi-C fragment" preparation. Chromatin digestion is carried out using restriction enzymes, with samples taken to check digestion efficiency. After that, the Hi-C sample is prepared by biotin labeling, blunt-end ligation, and DNA purification. DNA quality is checked, and if acceptable, the sample proceeds to the standard library construction workflow: Hi-C fragments are sonicated, end-repaired, A-tailed, and sequencing adapters are ligated to form adapter-ligated products with biotin enrichment. PCR optimization and amplification follow to obtain library products. A sample of the amplified library is taken for "Hi-C fragment ligation site quality control." If this passes, the entire library preparation is complete. |
WGS |
GENOMIC |
PCR |
PAIRED
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