| Accession | CRX1250717 |
| Organism | Mus musculus |
| Title | Active SETD4 hepatocytes2 |
| BioProject | PRJCA032048 |
| BioSample | SAMC4318289 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA was
purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was
carried out using divalent cations under elevated temperature in First Strand Synthesis
Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and
M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently
performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into
blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA
fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In
order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments
were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was
performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index
(X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was
assessed on the Agilent Bioanalyzer 2100 system. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
| Release date | 2024-11-09 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1365193 |
CRR1365193_r1.fq.gz
CRR1365193_r2.fq.gz
|
1,584.33
1,642.38
|
|
| Submitter | Xi zheng Jia (12107088@zju.edu.cn) |
|---|
| Organization | Zhe |
|---|
| Date submitted | 2024-11-06 |
|---|
