| Accession | CRX1269595 |
| Organism | Mus musculus |
| Title | W8_treat |
| BioProject | PRJCA032621 |
| BioSample | SAMC4397596 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The library preparation was conducted following the manufacturer’s instructions (10x Genomics). Initially, total cells were resuspended in the master mix and co-loaded with partitioning oil and gel beads. Subsequently, the poly-A RNA extracted from the cell lysate was reverse-transcribed into cDNA, incorporating an Illumina R1 primer sequence, a unique molecular identifier (UMI), and the 10x Barcode. The barcoded cDNA pool was then purified, PCR-amplified, and appropriately-sized fragments were selected using SPRIselect reagent for subsequent library construction. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2024-11-21 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1385345 |
CRR1385345_r1.fastq.gz
CRR1385345_r2.fastq.gz
|
1,959.01
2,044.21
|
|
| Submitter | Daqiang Song (cqmusdq@163.com) |
|---|
| Organization | The First Affiliated Hospital of Chongqing Medical University |
|---|
| Date submitted | 2024-11-21 |
|---|
