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文库构建方法 |
建库策略 |
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Select qualified high quality DNA samples (main tape >30kb); Randomly broken into segments (15-18kb) by Covaris ultrasonic crusher; Enrichment and purification of large DNA fragments by magnetic beads; Damage repair, terminal repair of fragmented DNA; Stem loop sequencing splices were connected at both ends of the DNA fragment, and exonuclease was used to remove the fragment that failed to connect. The constructed libraries were sequenced using the Pacbio Sequel II/Pacbio Sequel IIe platform. |
WGS |
GENOMIC |
unspecified |
SINGLE
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