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The cells were fixed with formaldehyde to cross-link DNA with proteins and between proteins, followed by enzyme digestion to produce sticky ends on both sides of the cross-linked sides, and then the end repair was carried out, biotin labeling was introduced, and the connection was performed. Decrosslinking, separating DNA from protein, protein from protein, extracting DNA, breaking it, capturing biotin-labeled fragments, building a library, and finally sequencing it on illumina. |
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